HELP : PCR from genomic DNA.

Susanne Rohrer srohrer at immv.unizh.ch
Fri Apr 14 09:10:25 EST 2000



jph at ibt.dtu.dk wrote:

> DNA from bacteria left at 4 C for weeks does not work when doing PCR. I get
> the band of interest AND smear upwards. The DNA contains garbage when checking
> on a gel.

My  bacterial DNA preps are good for at least a year at 4°C. Maybe you have a
contamination in it? Maybe the poblem lies in the PCR itself?

My advice would be to do a new prep. ( I know you didn't want to hear that one.)

Susanne





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