IPTG too early?

Kunal Mukhopadhyay kxm66 at psu.edu
Fri Apr 14 11:41:45 EST 2000


Hi
As with all experimentation another blooper occured in the lab -
BL21 cells were being grown to express protein. The expression is being
controlled by the lac operon.
So what went wrong? *IPTG was added to soon to the media. The cells are
still an hour away from log phase.
I was wondering how it would affect the expression of our protein.

Kunal

--


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What does it profit a man if he gains the world... but loses his soul?
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Kunal Mukhopadhyay

52, 152 Davey Lab,
Department Of Chemistry
Pennsylavania State University,
State College, PA 16802
USA






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