Help with pGL3 luciferase

Katherine Arden arden at plpk.uq.edu.au
Tue Apr 18 06:58:54 EST 2000


Hi,

I'm transfecting OK (opussum kidney) cells with a promoter cloned into
Promega's pGL3-Basic.  I'm getting variable levels of background from the
empty pGL3-Basic construct - sometimes very high luciferase counts.  Any
suggestions on what could be causing this?

If possible, please reply to the email address below.

Thanks


*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*^*
Katherine Arden, PhD
Department of Physiology and Pharmacology
University of Queensland
St Lucia, 4078
Australia

Ph:3365 2319
arden at plpk.uq.edu.au










More information about the Methods mailing list