Help with pGL3 luciferase

Katherine Arden arden at
Tue Apr 18 06:58:54 EST 2000


I'm transfecting OK (opussum kidney) cells with a promoter cloned into
Promega's pGL3-Basic.  I'm getting variable levels of background from the
empty pGL3-Basic construct - sometimes very high luciferase counts.  Any
suggestions on what could be causing this?

If possible, please reply to the email address below.


Katherine Arden, PhD
Department of Physiology and Pharmacology
University of Queensland
St Lucia, 4078

Ph:3365 2319
arden at

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