Not I question
css at med.unc.edu
Tue Apr 18 09:44:36 EST 2000
Is Not I a particularly tricky enzyme? It is behaving very
tempermentally. I've tested 2 different brands (Promega and Roche) with
2 different plasmids (1 plasmid that i purified, and one commercially
obtained plasmid). Interestingly, the only way I've been able to get
Not I to cut is to use the Roche buffer with the Promega enzyme!
So is Not I just weird, or am i the only one to ever have this problem?
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