Not I question

Caroline Szymeczek-Seay css at
Tue Apr 18 09:44:36 EST 2000

Is Not I a particularly tricky enzyme?  It is behaving very
tempermentally.  I've tested 2 different brands (Promega and Roche) with
2 different plasmids (1 plasmid that i purified, and one commercially
obtained plasmid).  Interestingly, the only way I've been able to get
Not I to cut is to use the Roche buffer with the Promega enzyme!  

So is Not I just weird, or am i the only one to ever have this problem?


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