RNA extraction after formaldehyde

Savita Shah spshah at stanford.edu
Mon Apr 24 11:23:58 EST 2000


Check the pH of your phenol .. basic pH might hydrolyze your RNA!!!

Luc.Ferrari at ctrmed.u-nancy.fr wrote:

> We performed RNA extraction on some tissu which have been perfused
> with formaldehyde.
> On a first attempt we get some RNA, less than with a fresh tissu.
> On all the other attempts we failed to get some RNA.
> We used the usual phenol protocol (adapted in a Promega kit).
>
> Is there any reason for our failure, or do we have to change our
> extraction protocol ? If so which protocol should we consider ?
>
> Luc





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