pcr conditions

Karen Roberts leehrat at mindspring.com
Mon Apr 24 16:58:31 EST 2000


Hi all,

I don't know a whole lot of pcr so this question is probably a little
basic.

How are pcr conditions determined?  the pcr rxns I have done in the past
always was someone giving me the cycling conditions.

Is there a formula of some kind?  I am having trouble on some pcr and
I'd like to address this potetial source.

TIA,
Karen





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