Non-radioactive end labeling of oligos

Chantale Lapierre ben_chantale at email.msn.com
Wed Apr 26 20:57:51 EST 2000


I agree, Roche's kit is very sensitive I have tried it myself.
Chantale
Dafydd <ddj at mrc-lmb.cam.ac.uk> wrote in message
news:3905AF19.EB2705F1 at mrc-lmb.cam.ac.uk...
> Have you thought about DIG labelled oligos? I have used them and they can
be
> very sensitive and Roche (which was Boehringer Mannheim) sell a kit for
gel
> shift assays - all you have to do is supply the protein and unlabelled
oligos.
> If you want more info go to :
>
>
http://biochem.roche.com/prodinfo_fst.htm?/prod_inf/manuals/dig_man/dig_toc.
htm
>
> Cheers,
>
> Dafydd
>
> --
> MRC Centre for Protein Engineering,
> Hills Road,                                      email:
ddj at mrc-lmb.cam.ac.uk
> Cambridge,
> http://www.mrc-cpe.cam.ac.uk/~ddj/
> CB2 2QH
>
>






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