DNA and RNA from ruminant feces (cattle, horses, sheep, etc) for PCR
hamel at cc.UManitoba.CA
Sat Apr 29 00:31:13 EST 2000
Does anyone have a good method for purifying _both_ DNA and RNA
PCR from ruminant feces (cattle, horses, sheep, etc) which does NOT
a commercial kit?
I am after DNA and RNA from bacteria, viruses, protozoan and
found in feces of cattle, horses, sheep (camels, muskox, deer, elk, etc,
I believe I have exhaustively tested all known methods (including
Yet still can only get slightly useable DNA & RNA ... most preps
ultimately have at
least some PCR inhibiting traits. Also, many preps yield DNA/RNA with
Please post replies to this newsgroup. I will post any emails I receive.
Scientist, Veterinary Services Lab, department of Agriculture,
Provincial government of Manitoba, Winnipeg, Manitoba, CANADA
More information about the Methods