Organic phase (was: Function of octanol/isoamylalcohol?)

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Tue Aug 1 23:07:10 EST 2000


At 08:26 AM 8/1/00 -0500, Chris LaRosa wrote:
>
>
>Jens Tiedemann wrote:
>> 
Not true!
Phenol-Chloroform, either together or by themselves are heavier than water.
The function of octanol or isoamyl alcohol is not what you suggest.



>
>You mean to the phenol??? Isoamyl alcohol changes the density or
>partitioning behavior of the organic phase, so that it centrifuges to
>the bottom of the tube and the aqueous phase remains on the top. 
>Otherwise, classic phenol/chloroform partitioning would be a hassle as
>the organic phase would be on top.
>
>
>
Dr. Hiranya Sankar Roychowdhury
Dept. of Molecular Biology	
PO Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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