Western

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Wed Aug 2 02:14:52 EST 2000



Kun Qian wrote:

> the primary antibody is rabbit anti
> rat, and the secondary antibody is sheep and rabbit IgG (H&L).

I sure hope this is a typo meaning sheep anti rabbit.

Since you say you get a dim band sometimes, check the dilutions of ab you're
using. If you have no background and the signal is specific, could you use a
bit more?

How are you detecting?







More information about the Methods mailing list