RNA from tuber tissue?

Frederik Börnke ricky_boernke at gmx.net
Wed Aug 2 01:36:29 EST 2000



"Allison B. MacKay" schrieb:
> 
> Here's _another_ plant RNA question for you all (what
> are the odds?):
> 
> I work on potato, and I want to purify RNA from tubers.
> I've used TRIzol and even with modifications (spinning
> down the initial homogenate, precipitating with high-
> salt instead of EtOH) I still get essentially no RNA
> and a thick pellet of a clear snot-like substance
> which I assume is polysaccharides.  Has anyone got a
> protocol for isolating RNA from starch-rich tissues
> like tuber?  Does potato tuber even _have_ much RNA?
> I eagerly await your advice :-)
> 
> --
> Allison Mackay
> Department of Biology
> Queen's University
> Kingston, ON

Hi,

we routinely use the method described by Logemann et al. (1987) for
isolation of RNA from all plant tissue. From tubers you might have
to include an additional LiCl precipitation step.

Ricky

 
*******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de






More information about the Methods mailing list