Function of octanol/isoamylalcohol?

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Wed Aug 2 10:00:47 EST 2000


I was told that IAA helps helps partition SDS out of the aqueous.  I don't
know about that.  I stopped using it several years ago, and never noticed a
difference!

Mike

>In article <3.0.6.32.20000801221251.007b32f0 at cnmailsvr.nmsu.edu>,
>hroychow at nmsu.edu ("Dr. Hiranya S. Roychowdhury") wrote:
>
>> The octanol or isoamyl alcohol prevents foaming of the organic phase.
>>
>> At 09:44 AM 8/1/00 +0200, Jens Tiedemann wrote:
>
>I agree with this comment, i.e. these components are unnecessary when a
>centrifuge is used to separate the phases or when they separate
>spontaneously, as they generally do in my DNA extractions. Foaming may
>have been a problem once; it rarely is now.
>
>--
>Bernie Cohen
>Division of Molecular Genetics
>University of Glasgow
>e-mail: b.l.cohen at bio.gla.ac.uk


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