Re-amplification of PCR product

Gregor Bucher st2153 at zi.biologie.uni-muenchen.de
Wed Aug 2 11:51:47 EST 2000


Hi Mike!

dilute the DNA 1:1000 or more (not more than 500 pg) and do only 20
cycles. Too much template leads to nonspecific bands. Another good idea is
to use nested primers.

Hope this helps,

Gregor

>HI, I try to re-amplify the product with the same first round pcr primers.
>Although I purified the first round pcr product with different method.
>Instead ofobtaining strong amplfied band I get weak band with lots of
>non-specific bands
>
>Thanks
>
>mike
>
>
>Sent via Deja.com http://www.deja.com/
>Before you buy.
-- 
Gregor Bucher, Zoologisches Institut der LMU München
0049/89/5902/444
st2153 at zi.biologie.uni-muenchen.de
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