Function of octanol/isoamylalcohol?
clarosa at biocomp.unl.edu
Wed Aug 2 18:28:31 EST 2000
"Bernard Murray, PhD" wrote:
> In article <3986CFF8.736090AA at biocomp.unl.edu>, Chris LaRosa
> <clarosa at biocomp.unl.edu> wrote:
> > You mean to the phenol??? Isoamyl alcohol changes the density or
> > partitioning behavior of the organic phase, so that it centrifuges to
> > the bottom of the tube and the aqueous phase remains on the top.
> > Otherwise, classic phenol/chloroform partitioning would be a hassle as
> > the organic phase would be on top.
Ok I was mistaken....Maybe I am thinking of chloroform only
extractions.... It was a long time ago but I thought the purpose was to
make the chloroform sink...
Confused and isolated in the midwestern wastelands.
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