Function of octanol/isoamylalcohol?

Chris LaRosa clarosa at biocomp.unl.edu
Wed Aug 2 18:28:31 EST 2000



"Bernard Murray, PhD" wrote:
> 
> In article <3986CFF8.736090AA at biocomp.unl.edu>, Chris LaRosa
> <clarosa at biocomp.unl.edu> wrote:
> 
> > You mean to the phenol??? Isoamyl alcohol changes the density or
> > partitioning behavior of the organic phase, so that it centrifuges to
> > the bottom of the tube and the aqueous phase remains on the top.
> > Otherwise, classic phenol/chloroform partitioning would be a hassle as
> > the organic phase would be on top.
> 
> 
Ok I was mistaken....Maybe I am thinking of chloroform only
extractions.... It was a long time ago but I thought the purpose was to
make the chloroform sink...

Confused and isolated in the midwestern wastelands.






More information about the Methods mailing list