SDS-PAGE gel resolution

Martin Thompson bugeater at cyllene.uwa.edu.au
Thu Aug 3 04:44:47 EST 2000


I was wondering if anyone knows what kind of factors influence how
'fuzzy' bands are on a SDS-PAGE gel.  Currently I am having problems
with this and would like to know how to increase the sharpness or
resolution of these bands.  I suspect that this is leading to problems
with me seeing exactly where my protein is (its expression is quite
low).
Thanks in advance,
Marty.
--
____________________________________
Martin J Thompson
Laboratory for Cancer Medicine
Level 6 MRF Building
Royal Perth Hospital
Perth WA 6001
Australia
Ph: 9224 0329
Fax: 9224 0322
email: bugeater at cyllene.uwa.edu.au



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