Ammonium precipitation of collagen

arjen a.vandenberg at amc.uva.nl
Thu Aug 3 07:56:27 EST 2000


I have an monoclonal antibody I would like to label with biotin. Before
I can do that I need to get  rid of gelatin that has been added as a
carrier protein in large amounts. I would like  to do this by
immunoprecipitation but I cannot find the concentration of ammonium
sulphate that precipitates gelatin (=collagen). Does anyone know where I
can find that?

cheers, arjen






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