SDS-PAGE gel resolution

Peter Pediaditakis pxpst2 at
Thu Aug 3 07:58:20 EST 2000

In article <39894C67.46145A64 at>, Oswaldo
<oswaldo at> wrote:

> Assuming that you are using right procedures and reagents for casting
> and running the gels, and that the reduction/denaturation of the samples
> is correct, I'd suggest to try concentrating the samples so you load the
> smallest volume possible. This usually produces much sharper bands.
> Amicon's Microcon (no affiliation) concentrating membranes with a 10.000
> Da cutoff are useful for this if your protein is heavier.

This is valid when running a continuous gel but less so when running a
discontinuous gel.

Peter Pediaditakis
University of Pittsburgh
Dept. of Pathology

remove "d-l-t" for reply

More information about the Methods mailing list