SDS-PAGE gel resolution

Peter Pediaditakis pxpst2 at dlt.pitt.edu
Thu Aug 3 07:58:20 EST 2000


In article <39894C67.46145A64 at iats.csic.es>, Oswaldo
<oswaldo at iats.csic.es> wrote:

> Assuming that you are using right procedures and reagents for casting
> and running the gels, and that the reduction/denaturation of the samples
> is correct, I'd suggest to try concentrating the samples so you load the
> smallest volume possible. This usually produces much sharper bands.
> Amicon's Microcon (no affiliation) concentrating membranes with a 10.000
> Da cutoff are useful for this if your protein is heavier.

This is valid when running a continuous gel but less so when running a
discontinuous gel.

-- 
Peter Pediaditakis
University of Pittsburgh
Dept. of Pathology

remove "d-l-t" for reply






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