Function of octanol/isoamylalcohol?

A.F. Simpson AFS7 at
Thu Aug 3 18:57:00 EST 2000

Chris LaRosa wrote:

> You mean to the phenol??? Isoamyl alcohol changes the density or
> partitioning behavior of the organic phase, so that it centrifuges to
> the bottom of the tube and the aqueous phase remains on the top.
> Otherwise, classic phenol/chloroform partitioning would be a hassle as
> the organic phase would be on top.

Are you aure?  My memory of phenol/chloroform extractions during
minipreps (without any isoamyl alcohol added) is that the aqueous phase
is on top.

Yep.  Just checked my old protocol.  No isoamyl alcohol, aqueous phase
on top.


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