Function of octanol/isoamylalcohol?
AFS7 at le.ac.uk
Thu Aug 3 18:57:00 EST 2000
Chris LaRosa wrote:
> You mean to the phenol??? Isoamyl alcohol changes the density or
> partitioning behavior of the organic phase, so that it centrifuges to
> the bottom of the tube and the aqueous phase remains on the top.
> Otherwise, classic phenol/chloroform partitioning would be a hassle as
> the organic phase would be on top.
Are you aure? My memory of phenol/chloroform extractions during
minipreps (without any isoamyl alcohol added) is that the aqueous phase
is on top.
Yep. Just checked my old protocol. No isoamyl alcohol, aqueous phase
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