pcr screening bacteria

felipe wettstein felipe.wettstein at ioez.tu-freiberg.de
Thu Aug 3 11:07:16 EST 2000

hi all

i have some 150 bacteria that i would like to screen, first only amplify
the 16SrDNA, and sequence, then look if a gen (where i have primers for)
is present. but i really would not like at all to make liquid cultures,
extract DNA, quantify and then pcr. with ecoli i usually took a little
bit from a colony and put it in the pcr reaction mix and denaturated for
some min at 96 C, and then added taq.
will this work also for example for gram positive bacteria? do you have
other ideas?
enjoy the lousy wheter and have a nice evening


-------| * ~. -felipe-wettstein----------------------------------------

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