{Q} Effective Inverse PCR?

[David Micklem]

In article <8mbf58$e4e$1 at nnrp1.deja.com>, Andreas Firzinger
>Never heard of such a unit-definition before, but
>you may be right. I had a look at the paper again. Authors did not
>explain their unit-definition, but they ligated cohesive ends
>(BamHI and BglII) and they used T4-DNA-ligase from New England
>Biolabs (NEB??).
>
>Ciao,
>
>Andreas:)


NEB = New England Biolabs = the best ligase available (IMO).

And yes, NEB use cohesive-end ligation units rather than Weiss, where 1
NEB unit = 0.015 Weiss. Measuring the activity of a ligase by its
ability to ligate DNA always seemed utterly sensible to me....


David

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