pcr screening bacteria

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Fri Aug 4 02:37:56 EST 2000



felipe wettstein wrote:

> with ecoli i usually took a little
> bit from a colony and put it in the pcr reaction mix and denaturated for
> some min at 96 C, and then added taq.
> will this work also for example for gram positive bacteria?

What we do with staph aureus: make a rather dense (McFarland 5) suspension
of cells in TE + 1% Triton X-100, boil for 10 min in waterbath, spin down
debris, and use about 1-2 microliters for PCR. I have done 120 strains like
that.






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