determine 5'-end of cDNA

Chris LaRosa clarosa at
Fri Aug 4 09:11:46 EST 2000

yang wrote:
> I am sure lots of experienced molecular biologist over there. Does
> anybody know a good method for determining 5'-end of cDNA?
> Right now I am using Primer Extension System (AMV reverse transciptase)
> from Promega. This kit works fine, but the sensitivity is not
> satisfactory, so lots of RNA needed for getting a visible band on PAGE
> gel. Because the amount of my RNA samples is limited, I am wondering if
> there is any improvements to this system or any other better kit for
> this kind of use. I will be grateful to any suggestions!
> Gen Y. Yang
> Department of Cell Biology
> ---
You can try 5' RACE procedures.   I used to like the kits from clontech.
Some like the kits from Lifetechnologies..... you can visit their
websites for details.

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