pcr dna purification for sequencing

Biopolymer Facility biopolymer at sc3101.med.buffalo.edu
Fri Aug 4 10:51:09 EST 2000


>hi everyone.
>
>how are you?
>
>my question is:
>
>what is the best method of purification of pcr products for sequencing?
>could you perhaps sketch the protocol briefly?
>
>hopefully someone will the take the time to help me out by answering this
>question.
>
>thank you very much:)
>
>alex

Hi Alex,
I work in a DNA sequencing core facility and I find that some of the best
results come from people who clean up their PCR reactions using an
exonuclease/shrimp alkaline phosphatase protocol. I don't have the protocol
in front of me but I believe it's relatively simple.

Michelle




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Biopolymer Facility
Roswell Park Cancer Institute
Buffalo, NY   14263
Phone: (716) 845-8032
Fax: (716) 845-7621
Email: biopolymer at sc3101.med.buffalo.edu
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