in vitro transcription

[ed_taboada at my-deja.com]

hi folks,

just a quick question !!!  if one of my primers has a built in T7
promoter, can I carry out in vitro transcription straight from the
amplicon ? or does it need extra bases at the end ?

thanks in advance,

Ed

ps. thanks to the group for the info on CSPD vs. CDP-star...wow...what a
world of difference !





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