in vitro transcription

ed_taboada at ed_taboada at
Fri Aug 4 13:11:54 EST 2000

hi folks,

just a quick question !!!  if one of my primers has a built in T7
promoter, can I carry out in vitro transcription straight from the
amplicon ? or does it need extra bases at the end ?

thanks in advance,


ps. thanks to the group for the info on CSPD vs. a
world of difference !

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