Electrophoresis problem

Peter Pediaditakis pxpst2 at dlt.pitt.edu
Fri Aug 4 18:57:24 EST 2000


In article <Pine.GSO.4.21.0008041710530.15751-100000 at rac5.wam.umd.edu>,
cxwang at wam.umd.edu (Chunxin Wang) wrote:

>  I have been having problems with my electrophoretic Western blot
> transfer. I use the transfer buffer of the following composition:
> 25mM Tris
> 192mM glycine
> 20% methanol

All sounds well for the trnsfer buffer.  I usually use a little SDS
(0.005%-.01%).
> 
> When I try to transfer, the BIO-RAD power supply switches from the
> constant voltage mode to a constant amperage mode automatically. The
> amperage is maximum(400mA) but the voltage is really low(~ 6-10V) I have
> tried using new Tris, Glycine, water, methanol, alternate power packs,
> alternate electrophoresis unit lids etc.. Nothing seems to ameliorate the
> problem. Could anyone please help? Thanks a ton.

Sounds like the power supply is your problem.  I am a lover of the good
old fashion switch and dial power supply.

-- 
Peter Pediaditakis
University of Pittsburgh
Dept. of Pathology

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