Gene specific primer for RT

ed_taboada at my-deja.com ed_taboada at my-deja.com
Sat Aug 5 18:14:48 EST 2000


In article <8mh478$pan$1 at justice.csc.cuhk.edu.hk>,
  "biobiobio_2000" <biobiobio_2000 at yahoo.com> wrote:
> Hi all,
> I need to design Gene-specific-primers for initiation of reverse
> transcription reaction.  How long are the primers normally required?
Is the
> design very different from those of PCR.  Any advise?
>
> biobiobio_2000
>

Your PCR primers should be able to prime cDNA synthesis fine. I have
occasionally encountered primers that didn't behave so well, but I'd say
that this is more the exception than the rule.

good luck !

Ed


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