How to screen subtracted library?
komala at hotmail.com
Sun Aug 6 00:42:14 EST 2000
Wonder if anyone can give me some advice. I have just completed constructing
a subtracted library and thinking of the best method to screen for
differentially expressed genes. I used the clontech PCR select kit and now
have about 1000 clones. Is it easier to just follow their recommendations or
is there an alternative way of screening. They have recommended using the
PCR products as probes (would it be enough?), for both subtracted and
unsubtracted controls. I am thinking of doing colony hybridisation since it
is much cheaper. I sure hope someone out there can help. Thanks.
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