RPA

[Nick Theodorakis]

"Curt Pfarr" <pfarr4 at home.com> wrote:
>Using an Ambion RPA kit....when we run out our RNA + probe w/o
RNAse we have
>a lot of counts hung in the well. When RNase treated this goes
away.
>Aggregates? Any ideas?
>

Just a guess... Maybe your template has a small amount of uncut
plasmid so RNA made from that just goes 'round and 'round and...

Nick



--
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu
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