{Q} Effective Inverse PCR?

Rich nospam at nopam.com
Tue Aug 8 15:44:48 EST 2000


 this means 300 units T4 ligase in one reaction!!
> >
I used their basic protocol and did my ligation in a 100ul volume with
50 units of T4 ligase. It worked well.

Cheers

Rich






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