Clone library reproducibility

David Kirchman kirchman at UDel.Edu
Wed Aug 9 13:26:39 EST 2000


If you make two clone libraries with the same method (same vector, etc)
with the same target DNA (or mRNA for a cDNA library), will you get the
"same" library?

We used two different DNA isolation methods to make two libraries of 16S
rRNA genes from one environmental sample.  The libraries differ in some
important aspects.  How much of the difference is due to uncontrolled
variability in making libraries?  We could repeat the experiment and
make two libraries with the same DNA isolation method, but that would be
a lot of work.  
 
**********************************************************************
David L. Kirchman                        302-645-4375                  
700 Pilottown Road                       302-645-4028 (fax)
College of Marine Studies                kirchman at udel.edu
University of Delaware 
Lewes, Delaware 19958 USA
http://www.ocean.udel.edu/faculty/kirchman/kirchman.htm


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