pd1 at mole.bio.cam.ac.uk
Sat Aug 12 08:33:10 EST 2000
Peter Loesh wrote:
> Hello !
> does anybody have a good experience with BNPS-skatole protein cleavage?
Don't know about "good". I was trying to modify Ws _without _ cleaving
the peptide backbone...didn't work and the damn stuff always fragmented.
> would very much like to know if residual BNPS-skatole interferes with
Not in my experience, at least after acetone precipitation
> and if it is possible to combine BNPS skatole and CNBr
> reactions in one. (I belive that these reagents react with eachother, but I
> am not a chemist).
Don't know I'm afraid!
A chemistry that is compatible with BNPS treatment
though, is formic acid cleavage of D-P bonds. 70% formic
acid o/n at 37 - 50 °C, IIRC.
Hope this helps
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