Methotrexate amplification?

Student mucineer at
Mon Aug 14 06:01:00 EST 2000

Dear All

I have a question which is puzzling me. After transfection and selection
(with media lacking ribo/deoxyribonucleosides and G418) of a CHO cell line
trasnfected with my plasmid of interest, it is possible to "amplify" the
copy number of the gene in the host chromosome by adding increasing amounts
of methotrexate.

Exactly how do these pieces of DNA which is already incorporated into the
host chromosome "increase in number"?

Any insights will be appreciated. Thanks.

More information about the Methods mailing list