mouse beta-actine PCR reaction

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Mon Aug 14 14:51:05 EST 2000


In article <399810A4.1028684F at bioch.unimaas.nl>,
  "Waander L. van Heerde" <WL.vanHeerde at bioch.unimaas.nl> wrote:
> Hallo,
> We do have a question about or beta-actine PCR. By using genomic DNA and
> primers, which are in the coding of mouse beta-actin, we do get two
> bands. Does somebody know whether beta-actin usually gives two bands and
> for what reason. Are their pseudogenes known or is the homology with
> other actin so high that this could be reason of it?  We have seen it
> several times.
>
> Thanks, Waander en Heidi
>
>

Mammalian genomes often contain several beta-actin pseudogenes and some
are even processed (no introns).

--
_______________________________________________
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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