Help: high mutation rate during RT-PCR

Gillen, Dr. Clemens Clemens.Gillen at grunenthal.de
Tue Aug 15 02:53:31 EST 2000


Hi everybody,
we are using the Advantage polymerase to amplify genes via RT-PCR and we
face the problems of various mutations. Do you know of certain experimental
conditions or better polymerases to overcome this problem?


Clemens Gillen

Dr. Clemens Gillen			Tel.:	+49-(0)241-569-2494	
Grünenthal GmbH			Fax.:	+49-(0)241-569-2789
Research & Development	email:Clemens.Gillen at grunenthal.de
Molecular Pharmacology		http://www.grunenthal.com		
Postfach 50 04 44	
D-52088 Aachen


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