Polynucleotide Kinase Reaction

schulffo Frank.Schulz at ruhr-uni-bochum.de
Wed Aug 16 06:29:18 EST 2000


Hi,
I want to label a trimeric oligodeoxynucleotide (CCG) on the 5'-End with
32P via a Polynucleotide Kinase Reaction with gamma-ATP.
Does anyone know, if this reacrion works well with a short
oligonucleotide and does anyone has an idea for the separation of the
reaction product from excess ATP and enzyme (I don't know if
precipitation works well with short oligonucleotides).

Thanks
Alex







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