Polynucleotide Kinase Reaction

schulffo Frank.Schulz at ruhr-uni-bochum.de
Wed Aug 16 06:29:18 EST 2000

I want to label a trimeric oligodeoxynucleotide (CCG) on the 5'-End with
32P via a Polynucleotide Kinase Reaction with gamma-ATP.
Does anyone know, if this reacrion works well with a short
oligonucleotide and does anyone has an idea for the separation of the
reaction product from excess ATP and enzyme (I don't know if
precipitation works well with short oligonucleotides).


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