CaMV35S promoter in animal cells

Jerry Ranch ranchjp at phibred.com
Wed Aug 16 10:08:39 EST 2000


I have found a cite for the transient functioning of CaMV35S in
Xenopus oocytes.

Efficient functioning of plant promoters and poly(A) sites in Xenopus
oocytes. 
Ballas N, Broido S, Soreq H, Loyter A 
Department of Biological Chemistry, Hebrew University, Jerusalem,
Israel.
Nucleic Acids Res 1989 Oct 11;17(19):7891-903

Mature Xenopus oocytes were challenged with DNA constructs including
plant regulatory elements, namely, the Cauliflower mosaic virus (CaMV)
35S promoter as well as the nopaline synthase (NOS) promoter and
polyadenylation signal. The bacterial chloramphenicol acetyl
transferase (CAT) was used as a reporter gene. When microinjected into
these cells, the plant-derived DNA constructs effectively promoted CAT
synthesis in a manner dependent on the presence of the plant promoters
and probably also on the polyadenylation signals. Structural studies
revealed that the supercoiled structures of the above DNA plasmids
were much more active in supporting CAT synthesis in microinjected
oocytes than their linear forms, with clear correlation between
efficient gene expression and DNA topology. In contrast, the linear
forms of these plasmids were considerably more active than the
supercoiled ones in transfected plant protoplasts. These findings
demonstrate, for the first time, the activity of regulatory elements
from plant genes in Xenopus oocytes and shed new light on the specific
rules applicable for gene expression in plant and animal cells




Can any provide a cite for the functioning of CaMV35S in STABLY
TRANSFORMED animal cells/organisms?


Thanks
Jerry






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