Need help on cell fractioning

Stephen Snowdy snowds at
Wed Aug 16 10:41:51 EST 2000

I'm fractioning cells (HeLa) into cyto vs nuclear fractions by hypotonic
treatment and dounce homogenization.  What I need is positive control
proteins for the nuclear and cyto fractions.  I was thinking histone H1
for the nuclear, but I'm not sure whether it comes out in the soluble
nuclear fraction or the insoluble.  Any ideas for the soluble cyto
Stephen Snowdy

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