thioated (spelling ?) oligos to supress 3'->5' exonuclease activity for long range PCR ?

Jorg Kirberg kirberg at bii.ch
Thu Aug 17 05:58:43 EST 2000


Well, the long subject line basically says it all:
thioated (spelling ?) oligos to supress 3'->5' exonuclease activity for
long range PCR ?

So, I wonder whether it is useful (and possible to make the oligo for
that) to make
the last base in an oligo (3'-base, the first to be synthesized, or
better, already present
on the cartrige) linked to the next base via a thioated phosphate group when
using the oligo for PCR with some proofreading (3'->5' exonuclease
activity) activity
in the polymerase(blent).

Since some physiciochemical characteristics might change (base pairing
???) this should
only be for the last (most 3') phosphodiesther bound, just enough to
prevent Pwo to gnaw
back the oligo.
BUT, does one gain a lot like this, based on the drawback that the oligo
somehow might
be changed ???

Any suggestions welcome.

jorg


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Joerg Kirberg                              Tel. 0041 - 61 - 605 12 70
Basel Institute for Immunology             FAX  0041 - 61 - 605 13 64
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