thioated (spelling ?) oligos to supress 3'->5' exonuclease activity for long range PCR ?

Dr. Duncan Clark Duncan at
Thu Aug 17 06:10:16 EST 2000

In article <kirberg-1708001256170001 at>, Jorg Kirberg
<kirberg at> writes
>BUT, does one gain a lot like this, based on the drawback that the oligo
>somehow might
>be changed ???

This was published maybe 4 years or so ago in NAR and worked well. I
know it was the first paper in one paricular issue but have no idea
offhand as to a reference.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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