thioated (spelling ?) oligos to supress 3'->5' exonuclease activity for long range PCR ?
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Thu Aug 17 06:10:16 EST 2000
In article <kirberg-1708001256170001 at 188.8.131.52>, Jorg Kirberg
<kirberg at bii.ch> writes
>BUT, does one gain a lot like this, based on the drawback that the oligo
>be changed ???
This was published maybe 4 years or so ago in NAR and worked well. I
know it was the first paper in one paricular issue but have no idea
offhand as to a reference.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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