thioated (spelling ?) oligos to supress 3'->5' exonuclease activity for long range PCR ?
rhubner at gins.uia.ac.be
Thu Aug 17 16:13:02 EST 2000
> This was published maybe 4 years or so ago in NAR and worked well. I
> know it was the first paper in one paricular issue but have no idea
> offhand as to a reference.
that for sure is the paper by A. Skerra from Nucleic Acids Res 1992
Jul25;20(14):3551-4... time flies...
Beware that the chemical modification (e.g. Beaucage reagent) is rather
difficult to perform and only completely modified oligos will perform as
indicated in this paper... [Of course, a lot of companies tell you they
can do this]
>Well, the long subject line basically says it all:
>So, I wonder whether it is useful (and possible to make the oligo for
In such LA blends the proofreading enzyme is "already" at a
comparatively low concentration...
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