Internal standards for RT-PCR

cppm suter un691cs at genius.embnet.dkfz-heidelberg.de
Fri Aug 18 10:35:43 EST 2000


On Fri, 18 Aug 2000, bovet wrote:

-David Humair wrote:
-
-> Hi netters,
-> I want to quantify the expression (in fact decrease) of an mRNA in
-> senescing plants. Regarding at some commercial kits (generally for
-> animal cells) people use: beta-actin, 18S RNA, GAPDH as standards. I
-> have also heard ubiquitin. Has anybody heard things about that. Does

-Dear Colleague,
-
-Lets try the protein 16S. It is an excellent constitutive gene for your
-application!
-
-Dr Lucien Bovet

Is it ? I thought 16s was regulated during development ?

Kind regards,                                Clemens Suter-Crazzolara






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