PCR problem

Riyaz Andrabi riyazandrabi at my-deja.com
Sun Aug 20 23:45:27 EST 2000


Hi
I used forward and reverse primers derived from conserved sequences
available in literature to hook out a particular gene.A single
amplified product of 1.4kb using both the primers together as well as
using the reverse primer alone was obtained, but no amplication was
obtained using the forward primer alone.I want to know whether it is
possible to hook out the full gene by using the single primer or can it
be a part of the gene or what?Thanks




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