4 fragment ligations.....?

Chris LaRosa clarosa at biocomp.unl.edu
Mon Aug 21 09:56:38 EST 2000


Recently there was a discussion on the feasiblility of complex
ligations.
I am contemplating attempting a 4 dna fragment ligation.  The constuct
will be directional with all sticky ends.   Any comments or references
would be appreciated. 

P. C. LaRosa
University of Nebraska, Lincoln NE.






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