Ca/Mg and RNA extraction

Tina anfortin at webnet.qc.ca
Tue Aug 22 19:06:05 EST 2000


Is there any effect of Ca and/or Mg ions on RNA extraction of human
neutrophils???
 I saw  in Maniatis in a old RNA extraction method that cultured cells needs
to be washed twice (to eliminate the culture medium) with ice-cold PBS
buffer without Ca and Mg ions before adding the extraction buffer.









More information about the Methods mailing list