Ca/Mg and RNA extraction

Tina anfortin at
Tue Aug 22 19:06:05 EST 2000

Is there any effect of Ca and/or Mg ions on RNA extraction of human
 I saw  in Maniatis in a old RNA extraction method that cultured cells needs
to be washed twice (to eliminate the culture medium) with ice-cold PBS
buffer without Ca and Mg ions before adding the extraction buffer.

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