Insert alone gives colonies?

Kristine E Gouveia Kristine.Gouveia at
Wed Aug 23 08:55:52 EST 2000

Dear Malay,
I am interested in obtaining more information on the problem with DH5alpha cells

and large plasmids.  I use DH5alpha cells on a regular basis when cloning and on
several occasions have run into a similar problem and Dr. Barrett in that I get
clones of the wrong size.  This you say is due to the DH5alpha cells causing
deletions in the plasmid.  What is the largest plasmid that would be well
maintained by DH5alpha cells?  Is there a reference on this?
Thank you very much,


More information about the Methods mailing list