Top Agar Newbie
ips98a at yahoo.co.uk
Thu Aug 24 14:47:24 EST 2000
1. Use agarose instead of agar.
2. Keep the plates for at least one hour at 4
degrees C, before plaque lifting.
If you still have problems, feel free to contact.
Bye for now,
--- Michael Herron <herro001 at maroon.tc.umn.edu> wrote:
> I have been doing some plaque lifts to screen a
> library and I have been
> having trouble with the top agar coming off
> Does anybody have any help hints to avoid this?
> / Michael J. Herron /
> / U of MN, Medicine/Infectious Diseases /
> / herro001 at umn.edu /
> / http://188.8.131.52 /
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