Top Agar Newbie

ip singh ips98a at yahoo.co.uk
Thu Aug 24 14:47:24 EST 2000


Dear Michael,
     1. Use agarose instead of agar. 
     2. Keep the plates for at least one hour at 4
degrees C, before plaque lifting. 
     If you still have problems, feel free to contact.
     Bye for now,
     IPSingh


--- Michael Herron <herro001 at maroon.tc.umn.edu> wrote:
> I have been doing some plaque lifts to screen a
> library and I have been
> having trouble with the top agar coming off
> sometimes.
> 
> Does anybody have any help hints to avoid this?
> 
> Thanks,
> Mike
> 
> 
> -- 
>  _________________________________________________
>      /  Michael J. Herron                        /
>     /   U of MN, Medicine/Infectious Diseases   /
>    /    herro001 at umn.edu                       /
>   /     http://128.101.243.213                /
>  /___________________________________________/
> 
> 


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