Top Agar Newbie
herro001 at maroon.tc.umn.edu
Thu Aug 24 15:52:01 EST 2000
I am using AgarOSE, and I let them sit in the coldroom overnite before removing.
What concentration of agarose are people using? (I am using 0.7%)
ip singh wrote:
> Dear Michael,
> 1. Use agarose instead of agar.
> 2. Keep the plates for at least one hour at 4
> degrees C, before plaque lifting.
> If you still have problems, feel free to contact.
> Bye for now,
> --- Michael Herron <herro001 at maroon.tc.umn.edu> wrote:
> > I have been doing some plaque lifts to screen a
> > library and I have been
> > having trouble with the top agar coming off
> > sometimes.
> > Does anybody have any help hints to avoid this?
> > Thanks,
> > Mike
> > --
> > _________________________________________________
> > / Michael J. Herron /
> > / U of MN, Medicine/Infectious Diseases /
> > / herro001 at umn.edu /
> > / http://220.127.116.11 /
> > /___________________________________________/
> Do You Yahoo!?
> Get your free @yahoo.co.uk address at http://mail.yahoo.co.uk
> or your free @yahoo.ie address at http://mail.yahoo.ie
/ Michael J. Herron /
/ U of MN, Medicine/Infectious Diseases /
/ herro001 at umn.edu /
/ http://18.104.22.168 /
More information about the Methods