Top Agar Newbie

Michael Herron herro001 at maroon.tc.umn.edu
Thu Aug 24 15:52:01 EST 2000


I am using AgarOSE, and I let them sit in the coldroom overnite before removing.

What concentration of agarose are people using?  (I am using 0.7%)

ip singh wrote:
> 
> Dear Michael,
>      1. Use agarose instead of agar.
>      2. Keep the plates for at least one hour at 4
> degrees C, before plaque lifting.
>      If you still have problems, feel free to contact.
>      Bye for now,
>      IPSingh
> 
> --- Michael Herron <herro001 at maroon.tc.umn.edu> wrote:
> > I have been doing some plaque lifts to screen a
> > library and I have been
> > having trouble with the top agar coming off
> > sometimes.
> >
> > Does anybody have any help hints to avoid this?
> >
> > Thanks,
> > Mike
> >
> >
> > --
> >  _________________________________________________
> >      /  Michael J. Herron                        /
> >     /   U of MN, Medicine/Infectious Diseases   /
> >    /    herro001 at umn.edu                       /
> >   /     http://128.101.243.213                /
> >  /___________________________________________/
> >
> >
> 
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-- 
 _________________________________________________
     /  Michael J. Herron                        /
    /   U of MN, Medicine/Infectious Diseases   /
   /    herro001 at umn.edu                       /
  /     http://128.101.243.213                /
 /___________________________________________/






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