GADPH as cytoplasmic marker

Stephen Snowdy snowds at med.unc.edu
Thu Aug 24 15:22:10 EST 2000


I fractionated cells by hypotonic buffer and dounce homogenization.  On
the advice (thank you for your help) of someone on this group, I used
GADPH as a positive control
for the cytoplasmic fraction.  Should the GADPH show up only
in the cyto fraction or will some also show up in the 
nuke fraction?  

Once again, thank you for your help.
-- 
Stephen Snowdy






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