Top Agar Newbie

[Rudi van de Wetering]

> >What concentration of agarose are people using?  (I am using 0.7%)
> >
> I rarely chilled the plates for more than an
> hour and I never had trouble with the top agarose peeling off.  I did
> always also make sure that my plates were a few days old before I used
> them.  I'm not sure whether this might be a factor also or not.
>
You have to be sure the plates have dried well enough. Fresh plates always
contain a lot of water.

Rudi