Top Agar Newbie

Rudi van de Wetering ReplyToButNoSpamR.vandewetering at
Fri Aug 25 03:03:52 EST 2000

> >What concentration of agarose are people using?  (I am using 0.7%)
> >
> I rarely chilled the plates for more than an
> hour and I never had trouble with the top agarose peeling off.  I did
> always also make sure that my plates were a few days old before I used
> them.  I'm not sure whether this might be a factor also or not.
You have to be sure the plates have dried well enough. Fresh plates always
contain a lot of water.


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