Top Agar Newbie

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Fri Aug 25 09:15:16 EST 2000


In addition to what the others say (use agarose, chill, use older
plates), we found it helpful to prewet the membranes in water, then
allow them to airdry on blotting paper for several minutes. They should
still be moist when putting them onto the plate.

Frank





Michael Herron wrote:
> 
> I have been doing some plaque lifts to screen a library and I have been
> having trouble with the top agar coming off sometimes.
> 
> Does anybody have any help hints to avoid this?
> 
> Thanks,
> Mike
> 
> --
>  _________________________________________________
>      /  Michael J. Herron                        /
>     /   U of MN, Medicine/Infectious Diseases   /
>    /    herro001 at umn.edu                       /
>   /     http://128.101.243.213                /
>  /___________________________________________/






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